Test kit for detection of Feline Corona Virus Antigen

BIOPLACEFLOW FCoV Ag

INTENDED USE
BIOPLACEFLOW FCoV Ag is a one-step qualitative immunochromatographic assay for the
detection Feline Corona Virus antigen in the cat’s feces or vomit, to provide a reference for
diagnosis of Feline Infectious Peritonitis (FIP) infection.
Assay Time: 5-10 minutes
PRINCIPLE
The BIOPLACEFLOW FCoV Ag kit is based on sandwich lateral flow immunochromatographic
assay. The test device has a testing window for the observation of assay running and result
reading. The testing window has an invisible test (T) zone and a control (C) zone before
running the assay. When the treated sample was applied into the sample hole on the device,
the liquid will laterally flow through the surface of the test strip and react with the pre-coated
monoclonal antibodies. If there is FCoV antigen in the specimen, a visible T line will appear.
The C line should always appear after a sample is applied, which indicates a valid result. By
this means, the device can accurately indicate the presence of Feline Corona Virus antigen in
the specimen.
REAGENTS AND MATERIALS
- Test device, with disposable dropper
- Assay buffer
- Cotton swab
- Product Manual
STORAGE AND STABILITY
The kit can be stored at room temperature (8-25°C). The test kit is stable through the
expiration date (12 months) marked on the package label. DO NOT FREEZE. Do not store the
test kit in direct sunlight.
TEST PROCEDURE
- Collect cat’s fresh feces or vomit with the cotton swab from cat’s anus or from the
ground.
- Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample
extraction.
- Take out the test device from the foil pouch and place it horizontally.

- Suck the treated sample extraction from the assay buffer tube and add 3 drops into the
sample hole (S) of the test device.
- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Note: If the liquid will not flow through the surface of the test strip in 30 seconds, please
add another drop of the treated sample extraction.

INTERPRETATION OF RESULTS
- Positive: The presence of both (C) line and zone (T) line, no matter T line is clear or
vague.
- Negative: Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.
PRECAUTIONS
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not use the test beyond its expiration date.
- The components in this kit have been quality control tested as standard batch unit. Do
not mix components from different lot numbers.
- All specimens are of potential infection. It must be strictly treated according to the rules
and regulations by local states.
LIMITATION
Test kit for detection of Feline Corona Virus Antigen BIOPLACEFLOW FCoV Ag is for in vitro
veterinary diagnosis use only. All result should be considered with other clinical information
available with veterinarian. It is suggested to apply a further confirmative method such as
RT-PCR when positive result was observed.